KMID : 0811720170210040449
|
|
Korean Journal of Physiology & Pharmacology 2017 Volume.21 No. 4 p.449 ~ p.456
|
|
Beauvericin, a cyclic peptide, inhibits inflammatory responses in macrophages by inhibiting the NF-¥êB pathway
|
|
Yoo Sul-Gi
Kim Mi-Yeon Cho Jae-Youl
|
|
Abstract
|
|
|
Beauvericin (BEA), a cyclic hexadepsipeptide produced by the fungus Beauveria bassiana, is known to have anti-cancer, anti-inflammatory, and anti-microbial actions. However, how BEA suppresses macrophage-induced inflammatory responses has not been fully elucidated. In this study, we explored the anti-inflammatory properties of BEA and the underlying molecular mechanisms using lipopolysaccharide (LPS)-treated macrophage-like RAW264.7 cells. Levels of nitric oxide (NO), mRNA levels of transcription factors and the inflammatory genes inducible NO synthase (iNOS) and interleukin (IL)-1, and protein levels of activated intracellular signaling molecules were determined by Griess assay, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), luciferase reporter gene assay, and immunoblotting analysis. BEA dose-dependently blocked the production of NO in LPS-treated RAW264.7 cells without inducing cell cytotoxicity. BEA also prevented LPS-triggered morphological changes. This compound significantly inhibited nuclear translocation of the NF-¥êB subunits p65 and p50. Luciferase reporter gene assays demonstrated that BEA suppresses MyD88-dependent NF-¥êB activation. By analyzing upstream signaling events for NF-¥êB activation and overexpressing Src and Syk, these two enzymes were revealed to be targets of BEA. Together, these results suggest that BEA suppresses NF-¥êB-dependent inflammatory responses by suppressing both Src and Syk.
|
|
KEYWORD
|
|
Anti-inflammatory activity, Beauvericin, NF-¥êB, Src, Syk
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|